Journal: bioRxiv

Article Title: Functional and transcriptomic analyses in Neurospora crassa reveal the crucial role of N-glycoprotein deglycosylation process in fungal homeostasis

doi: 10.64898/2026.03.25.714127

Figure Lengend Snippet: Deletion of the gh18-10 ENGase increases Neurospora crassa tolerance to variable stress conditions. ( A ) Representative colony morphology of Neurospora crassa wild type (WT), Δ gh18-10 , and Δ pngA strain grown on solid VM medium under control conditions or supplemented with caffeine (cell wall stress), CoCl₂ (hypoxia), DTT (ER stress), H₂O₂ (oxidative stress), or tunicamycin (ER stress). ( B ) Mycelia growth was quantified as mycelial diameter (cm) under each condition (right) 24 hours post inoculation at 30°C. Bars represent the mean ± error. Asterisks indicate statistically significant differences relative to WT ( p < 0.05) according to Tukey test.

Article Snippet: Statistical analysis was performed using one-way ANOVA using the Tukey test (p < 0.05) (Minitab, LLC, 2021).

Techniques: Control

Journal: bioRxiv

Article Title: Functional and transcriptomic analyses in Neurospora crassa reveal the crucial role of N-glycoprotein deglycosylation process in fungal homeostasis

doi: 10.64898/2026.03.25.714127

Figure Lengend Snippet: The Neurospora crassa nag-1 gene coding for a putative GH20 N-acetyl hexosaminidase is uniquely induced on gh18-10 deletion strain upon ER stress. (A ) Venn diagram of differentially expressed genes (DEGs) of Δ pngA , Δ gh18-10 and WT strain grown under tunicamycin ER stress conditions. ( B ) Predicted schematic overview and structural comparison highlighting similarities between the nag-1 and gh18-10 enzymes. PDB structures were generated using AlphaFold 3, and structural alignments were performed with TM-align using default parameters. ( C ) Phenotypic analysis of nag-1 deletion strain under tunicamycin stress conditions. Mycelial radial growth was measured 24 h of incubation at 30 °C. Different letters (a, b, c) indicate statistically significant differences, and bars represent the mean ± error. Asterisks indicate statistically significant differences ( p < 0.05) according to Tukey test.

Article Snippet: Statistical analysis was performed using one-way ANOVA using the Tukey test (p < 0.05) (Minitab, LLC, 2021).

Techniques: Comparison, Generated, Incubation